US 12,176,066 B2
Target-associated molecules for characterization associated with biological targets
David Tsao, San Carlos, CA (US); and Oguzhan Atay, Menlo Park, CA (US)
Assigned to BILLIONTOONE, INC., Menlo Park, CA (US)
Filed by BillionToOne, Inc., Menlo Park, CA (US)
Filed on Mar. 24, 2023, as Appl. No. 18/189,632.
Application 18/189,632 is a continuation of application No. 16/055,889, filed on Aug. 6, 2018, granted, now 11,646,100.
Claims priority of provisional application 62/541,555, filed on Aug. 4, 2017.
Prior Publication US 2023/0268025 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G16B 20/00 (2019.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6827 (2018.01); G01N 33/68 (2006.01); G16B 5/00 (2019.01); G16B 25/00 (2019.01); G16B 30/00 (2019.01); G16B 50/00 (2019.01)
CPC G16B 20/00 (2019.02) [C12N 15/10 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6827 (2013.01); G01N 33/689 (2013.01); G16B 5/00 (2019.02); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 50/00 (2019.02); C12Q 2600/166 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for determining an abundance of a biological target from a biological sample, the method comprising:
providing a set of target-associated molecules comprising target-associated regions with sequence similarity to a target sequence region of the biological target;
generating at least one spike-in mixture, wherein the generation of the at least one spike-in mixture comprises amplification of the set of target-associated molecules and nucleic acid molecules from the biological sample, the nucleic acid molecules being associated with the target sequence region;
sequencing the at least one spike-in mixture to generate sequencing results; and
determining the abundance of the biological target based on the sequencing results.