US 12,174,194 B2
Tumor marker, and method for collecting and detecting tumor cell in distinction from contaminant cell
Yuichi Kumaki, Kanagawa (JP); Sayaka Hirai, Kanagawa (JP); Makoto Otsuki, Kanagawa (JP); Daisuke Miki, Kanagawa (JP); and Toru Futami, Kanagawa (JP)
Assigned to TOSOH CORPORATION, Yamaguchi (JP)
Appl. No. 16/978,887
Filed by TOSOH CORPORATION, Yamaguchi (JP)
PCT Filed Feb. 27, 2019, PCT No. PCT/JP2019/007470
§ 371(c)(1), (2) Date Sep. 8, 2020,
PCT Pub. No. WO2019/172030, PCT Pub. Date Sep. 12, 2019.
Claims priority of application No. 2018-042710 (JP), filed on Mar. 9, 2018; application No. 2018-105966 (JP), filed on Jun. 1, 2018; and application No. 2018-193273 (JP), filed on Oct. 12, 2018.
Prior Publication US 2020/0408769 A1, Dec. 31, 2020
Int. Cl. G01N 33/574 (2006.01); C07K 14/47 (2006.01); C07K 14/705 (2006.01); C07K 14/745 (2006.01); C12Q 1/6886 (2018.01)
CPC G01N 33/57492 (2013.01) [C07K 14/4725 (2013.01); C07K 14/705 (2013.01); C07K 14/70578 (2013.01); C07K 14/745 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/158 (2013.01)] 6 Claims
 
1. A method of detecting a tumor cell contained in a sample in distinction from a contaminant cell, the method comprising detecting one or more polypeptides selected from the group consisting of the following (i) to (iii) present in the sample:
(i) a polypeptide comprising at least the amino acid sequence of any of SEQ ID NOs: 1 to 3;
(ii) a polypeptide comprising an amino acid sequence having a homology of not less than 85% to the amino acid sequence of any of SEQ ID NOs: 1 to 3; and
(iii) a polypeptide comprising at least a splicing variant of the amino acid sequence of any of SEQ ID NOs: 1 to 3, or a polypeptide comprising at least a splicing variant of a polypeptide having a homology of not less than 85% to the amino acid sequence of any of SEQ ID NOs: 1 to 3, and
wherein the tumor cell is an EpCAM (Epithelial Cell Adhesion Molecule)-negative tumor cell.