US 12,173,366 B2
DNA sequencing by synthesis using modified nucleotides and nanopore detection
Jingyue Ju, Englewood Cliffs, NJ (US); Shiv Kumar, Belle Mead, NJ (US); Zengmin Li, Flushing, NY (US); Chuanjuan Tao, New York, NY (US); Minchen Chien, Tenafly, NJ (US); James J. Russo, New York, NY (US); Sergey Kalachikov, New York, NY (US); Ken Shepard, Ossining, NY (US); and Jacob Karl Rosenstein, Providence, RI (US)
Assigned to THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US)
Filed by The Trustees of Columbia University in the City of New York, New York, NY (US)
Filed on May 5, 2022, as Appl. No. 17/737,721.
Application 17/737,721 is a division of application No. 16/653,278, filed on Oct. 15, 2019, granted, now 11,499,186.
Application 16/653,278 is a division of application No. 13/994,431, granted, now 10,443,096, issued on Oct. 15, 2019, previously published as PCT/US2011/065640, filed on Dec. 16, 2011.
Claims priority of provisional application 61/557,558, filed on Nov. 9, 2011.
Claims priority of provisional application 61/424,480, filed on Dec. 17, 2010.
Prior Publication US 2023/0010731 A1, Jan. 12, 2023
Int. Cl. C07H 19/10 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); G01N 33/487 (2006.01)
CPC C12Q 1/6869 (2013.01) [C07H 19/10 (2013.01); C07H 21/00 (2013.01); G01N 33/48721 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A compound having the structure:

OG Complex Work Unit Chemistry
wherein the tag is polyethylene glycol, an amino acid, a carbohydrate, mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide or hexanucleotide, wherein R1 is OH, wherein R2 is H or OH, wherein X is O, NH, S or CH2, wherein the base is adenine, guanine, cytosine, thymine, uracil, a 7-deazapurine or a 5-methylpyrimidine, and wherein n is 1, 2, 3, or 4.