	US 12,173,348 B2
	Method for detecting enzymatic reaction product
Kazuhito Tabata, Tokyo (JP); Yoshihiro Minagawa, Tokyo (JP); and Hiroyuki Noji, Tokyo (JP)
Assigned to Japan Science and Technology Agency, Saitama (JP)
Appl. No. 16/976,399
Filed by Japan Science and Technology Agency, Saitama (JP)
PCT Filed Mar. 4, 2019, PCT No. PCT/JP2019/008411 § 371(c)(1), (2) Date Aug. 27, 2020, PCT Pub. No. WO2019/168200, PCT Pub. Date Sep. 6, 2019.
Claims priority of application No. 2018-037085 (JP), filed on Mar. 2, 2018.
Prior Publication US 2020/0407769 A1, Dec. 31, 2020
Int. Cl. C12Q 1/34 (2006.01); C12Q 1/70 (2006.01)

CPC C12Q 1/34 (2013.01) [C12Q 1/70 (2013.01); C12Y 302/01 (2013.01); G01N 2333/924 (2013.01)]

15 Claims

1. A method for detecting a reaction product, comprising reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and

wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO), and wherein the concentration of the buffering substance in the hydrophilic solvent is at least 500 mM.