US 12,173,326 B2
Cell culture media and methods for generating human alveolar macrophage-like cells
Larry S. Schlesinger, San Antonio, TX (US); and Susanta Pahari, San Antonio, TX (US)
Assigned to TEXAS BIOMEDICAL RESEARCH INSTITUTE, San Antonio, TX (US)
Filed by TEXAS BIOMEDICAL RESEARCH INSTITUTE, San Antonio, TX (US)
Filed on Mar. 30, 2022, as Appl. No. 17/657,344.
Claims priority of provisional application 63/200,810, filed on Mar. 30, 2021.
Prior Publication US 2022/0315900 A1, Oct. 6, 2022
Int. Cl. C12N 5/071 (2010.01)
CPC C12N 5/0688 (2013.01) [C12N 2501/15 (2013.01); C12N 2501/22 (2013.01); C12N 2501/231 (2013.01); C12N 2506/115 (2013.01)] 9 Claims
 
1. A method of generating human alveolar macrophage-like (AML) cells in-vitro from human blood-derived monocytes, the method comprising:
culturing a population of human blood-derived monocytes in a cell culture medium containing a mixture of a surfactant and two or more cytokines to generate the AML cells, wherein the human AML cells have increased expression of one or more of MRC1 (CD206), MARCO, MERTK, CES1, CD170, MCL1, CCL18, CXCL3, DUSP1, CXCL5, PU.1, H3K4mel, CD200R, CD11c, CD163, MCEMP1, and/or decreased expression of one or more of CD11b, CD36, MMP7, MMP9, H3K4me3 as compared to the human blood-derived monocytes.