	US 12,173,322 B2
	Genome-edited induced pluripotent stem cells, and cells derived therefrom, and uses thereof
Nathan Palpant, St. Lucia (AU); and Meredith Ann Redd, St. Lucia (AU)
Assigned to THE UNIVERSITY OF QUEENSLAND, St Lucia (AU)
Filed by The University of Queensland, St. Lucia (AU)
Filed on Dec. 9, 2020, as Appl. No. 17/116,332.
Prior Publication US 2022/0177846 A1, Jun. 9, 2022
Int. Cl. C12N 5/077 (2010.01); A61K 45/06 (2006.01); A61P 9/10 (2006.01)

CPC C12N 5/0657 (2013.01) [A61K 45/06 (2013.01); A61P 9/10 (2018.01)]

6 Claims

1. A method of producing a transplant composition comprising cardiomyocytes having a ASIC1a−/− phenotype, the method comprising the steps of:

a) genetically modifying stem cells to have a ASIC1a−/− phenotype;

b) differentiating said stem cells having a ASIC1a−/− phenotype to cardiomyocytes, wherein the differentiation is performed in vitro; and

c) admixing said cardiomyocytes with a pharmaceutically acceptable carrier.