US 12,173,262 B2
Devices comprising organoid chambers and uses thereof to culture, maintain, monitor or test organoids
Kevin D. Costa, Hong Kong (HK); Yosuke Kurokawa, Hong Kong (HK); Eugene K. Lee, Hong Kong (HK); David D. Tran, Hong Kong (HK); and Bernard Fermini, Hong Kong (HK)
Assigned to Novoheart International Limited, Kowloon (HK)
Filed by Novoheart International Limited, Kowloon (HK)
Filed on Jun. 30, 2021, as Appl. No. 17/364,288.
Claims priority of provisional application 63/046,435, filed on Jun. 30, 2020.
Prior Publication US 2021/0403844 A1, Dec. 30, 2021
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12N 5/071 (2010.01)
CPC C12M 21/08 (2013.01) [C12M 23/22 (2013.01); C12M 23/34 (2013.01); C12N 5/0602 (2013.01); C12N 2513/00 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A bioreactor for an organoid or tissue derived from a hollow organ comprising:
an organoid chamber comprising an organoid or tissue including:
(a) a top layer configured to direct fluid flow into and/or out of the organoid chamber;
(b) a middle layer comprising a point of attachment for the organoid chamber; and
(c) a bottom layer comprising a reservoir for culture fluid;
wherein the top layer and middle layer are in fluid communication capable of exchanging an internal fluid,
wherein the top layer, middle layer and bottom layer are in fluid communication capable of exchanging an external fluid;
wherein the top layer, middle layer and bottom layer include fluid channels that are connected using at least one Luer connection; and
wherein the organoid chamber comprises an elastomeric material providing a barrier between the internal fluid and the external fluid wherein the elastomeric material is a balloon.