	US 12,173,049 B2
	Cell culture medium
Christopher T. Leber, San Diego, CA (US); Michael W. Y. Shen, San Diego, CA (US); Yiwen Tao, San Diego, CA (US); and Hugh Eugene Murray, IV, San Diego, CA (US)
Assigned to La Jolla Biologics, Inc., San Diego, CA (US)
Filed by La Jolla Biologics, Inc., San Diego, CA (US)
Filed on Jun. 19, 2020, as Appl. No. 16/906,766.
Application 16/906,766 is a division of application No. 15/498,221, filed on Apr. 26, 2017, granted, now 10,703,800.
Claims priority of provisional application 62/327,964, filed on Apr. 26, 2016.
Prior Publication US 2020/0385444 A1, Dec. 10, 2020
Int. Cl. C07K 16/00 (2006.01); C12N 5/00 (2006.01); C12P 21/00 (2006.01)

CPC C07K 16/00 (2013.01) [C12N 5/0018 (2013.01); C12P 21/005 (2013.01); C07K 2317/14 (2013.01); C07K 2317/41 (2013.01); C07K 2317/73 (2013.01); C12N 2500/12 (2013.01); C12N 2500/30 (2013.01); C12N 2500/36 (2013.01); C12N 2510/02 (2013.01)]

19 Claims

1. A method for producing one or more recombinant polypeptides from a genetically engineered Chinese Hamster Ovary (CHO) cell, the method comprising:

(a) culturing said engineered CHO cell in a medium under suitable conditions for the production of said one or more recombinant polypeptides, and increasing the concentration of ethanol in the medium during the culturing, wherein the concentration of ethanol is increased daily by 0.01% to 0.15% (v/v); and

(b) producing said one or more recombinant polypeptides,

wherein the medium comprises

(i) a basal medium or a feed medium; and

(ii) ethanol,

wherein the genetically engineered CHO cell is a CHO.DXB11 cell or a CHO.DG44 cell.