	US 12,173,032 B2
	Recombinant rotavirus expression system and recombinant rotaviruses
John Thomas Patton, Bloomington, IN (US); and Asha Ann Philip, Bloomington, IN (US)
Assigned to The Trustees of Indiana University, Bloomington, IN (US)
Appl. No. 17/259,844
Filed by The Trustees of Indiana University, Bloomington, IN (US)
PCT Filed Jul. 12, 2019, PCT No. PCT/US2019/041677 § 371(c)(1), (2) Date Jan. 12, 2021, PCT Pub. No. WO2020/014654, PCT Pub. Date Jan. 16, 2020.
Claims priority of provisional application 62/697,869, filed on Jul. 13, 2018.
Prior Publication US 2021/0269488 A1, Sep. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/005 (2006.01); A61K 39/15 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)

CPC C07K 14/005 (2013.01) [A61K 39/15 (2013.01); C12N 7/00 (2013.01); A61K 2039/5254 (2013.01); C12N 2720/12321 (2013.01); C12N 2720/12322 (2013.01); C12N 2720/12334 (2013.01); C12N 2720/12351 (2013.01)]

17 Claims

1. A recombinant rotavirus comprising a gene segment including a nucleotide sequence encoding an intact, full-length rotavirus nonstructural protein 3 (NSP3) and a nucleotide sequence encoding a non-rotavirus polypeptide, wherein the NSP3 and the non-rotavirus polypeptide are encoded by a single open reading frame, separated by a self-cleavage protease domain.