US 12,172,378 B1
Method and system of controlling cell functions on 3D printed and 3D manufactured materials modified with lipases
Thomas Webster, Lviv, RI (US); Christopher Dang, Berkeley, CA (US); Aditya Prakash Morey, Freemont, CA (US); and Dylan Tepper, Hayward, CA (US)
Assigned to Zeda Holdings, Inc., Fremont, CA (US)
Filed by PrinterPrezz, Inc., Fremont, CA (US)
Filed on Nov. 15, 2021, as Appl. No. 17/526,648.
Int. Cl. B28B 1/00 (2006.01); B28B 11/00 (2006.01); B28B 11/22 (2006.01); B29C 64/147 (2017.01); B29C 64/30 (2017.01); B29C 64/35 (2017.01); B33Y 10/00 (2015.01); B33Y 40/20 (2020.01); B29L 31/00 (2006.01); B33Y 80/00 (2015.01)
CPC B29C 64/30 (2017.08) [B28B 1/001 (2013.01); B28B 11/00 (2013.01); B28B 11/22 (2013.01); B29C 64/147 (2017.08); B29C 64/35 (2017.08); B33Y 10/00 (2014.12); B33Y 40/20 (2020.01); B29L 2031/7532 (2013.01); B33Y 80/00 (2014.12)] 17 Claims
OG exemplary drawing
 
1. A method for creating a 3D printed implantable device having a surface that promotes bone ingrowth and inhibits infection comprising:
a. fusing a plurality of parallel planar layers of material to fabricate the 3D printed implantable device;
b. exposing the 3D printed implantable device to a lipase solution consisting of lipase and water to etch a nanoparticle layer on the surface of the 3D printed implantable device having nanoscale surface features that increase a surface area of the 3D printed implantable device and increase the surface energy of the surface of the 3D printed implantable device; and
c. rinsing the 3D printed implantable device with an acetone cleaning solution to stop the etching of the 3D printed implantable device by the lipase solution.