US 12,172,158 B2
Microfluidic device and method of manufacturing the same
Jeff Hawkins, San Diego, CA (US); Ian Kleinemolen, San Diego, CA (US); Florence Lee, San Diego, CA (US); Matthew Tyson Grant, San Diego, CA (US); Dena Marrinucci, San Diego, CA (US); and Xiaodong Zhao, San Diego, CA (US)
Assigned to Truvian Sciences, Inc., San Diego, CA (US)
Filed by Truvian Sciences, Inc., San Diego, CA (US)
Filed on Dec. 13, 2023, as Appl. No. 18/539,226.
Application 18/539,226 is a continuation of application No. PCT/US2022/034722, filed on Jun. 23, 2022.
Claims priority of provisional application 63/214,615, filed on Jun. 24, 2021.
Prior Publication US 2024/0139732 A1, May 2, 2024
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502707 (2013.01) [B01L 2300/0645 (2013.01); B01L 2300/0887 (2013.01)] 20 Claims
 
1. A method of manufacturing a microfluidic device comprising:
providing a substrate layer;
providing an adhesive layer comprising a curable adhesive, the adhesive layer having a first thickness;
providing a top layer;
forming at least one channel or recess feature in the adhesive layer;
laminating the adhesive layer and the top layer together thereby forming an intermediate laminate;
bonding the intermediate laminate to the substrate layer thereby forming a composite laminate, wherein the adhesive layer of the composite laminate has a second thickness; and
curing the composite laminate to form the microfluidic device, wherein curing comprises treating the composite laminate to promote polymerization of a monomer causing transition of the adhesive layer from the second thickness to a stable and uniform third thickness, and wherein the cured adhesive layer has a stable and uniform thickness across an entire width and length of the device, the stable and uniform thickness having a thickness deviation of less than about 5 μm.