US 12,171,907 B2
Infused demineralized bone fibers
Timothy Ganey, Tampa, FL (US); Tracy Scott Anderson, Atlanta, GA (US); and Harry Thomas Temple, Miami, FL (US)
Assigned to VIVEX BIOLOGICS GROUP, INC., Atlanta, GA (US)
Filed by Vivex Biologics Group, Inc., Atlanta, GA (US)
Filed on Aug. 6, 2021, as Appl. No. 17/395,745.
Application 17/395,745 is a division of application No. 15/999,087, filed on Aug. 17, 2018, granted, now 11,116,872.
Prior Publication US 2021/0361828 A1, Nov. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61L 27/38 (2006.01); A61B 17/16 (2006.01); A61L 27/36 (2006.01); A61L 27/50 (2006.01); A01N 1/02 (2006.01); A61L 27/24 (2006.01)
CPC A61L 27/3608 (2013.01) [A61B 17/1635 (2013.01); A61L 27/3604 (2013.01); A61L 27/3683 (2013.01); A61L 27/50 (2013.01); A01N 1/0221 (2013.01); A01N 1/0284 (2013.01); A01N 1/0289 (2013.01); A61L 27/24 (2013.01); A61L 2430/02 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of making infused at least partially demineralized or at least partially decalcified bone fibers, comprising:
(a) cutting or shaving whole bone into bone fibers;
(b) washing the bone fibers;
(c) at least partially demineralizing or at least partially decalcifying the bone fibers, creating a surface demineralization or decalcification that extends, into the bone fibers; and
(d) infusing the at least partially demineralized or at least partially decalcified bone fibers with a supernatant of biologic material and a polyampholyte cryoprotectant to create infused at least partially demineralized or at least partially decalcified bone fibers, wherein infusing comprises one or more of:
i. exposing the at least partially demineralized or at least partially decalcified bone fibers, supernatant of biologic material, and polyampholyte cryoprotectant to a negative pressure or vacuum to draw the supernatant and polyampholyte cryoprotectant into the bone fibers; and
ii. exposing the at least partially demineralized or at least partially decalcified bone fibers, supernatant of biologic material, and polyampholyte cryoprotectant to a positive pressure to drive the supernatant and polyampholyte cryoprotectant into the bone fibers.