US 11,852,639 B2
Microfluidic chip-based, universal coagulation assay
Sasha Bakhru, Danbury, CT (US); Bryan Laulicht, Danbury, CT (US); Stefan Zappe, Danbury, CT (US); and Solomon Steiner, Danbury, CT (US)
Assigned to Perosphere Technologies Inc., Danbury, CT (US)
Filed by Perosphere Technologies Inc., Danbury, CT (US)
Filed on Feb. 2, 2022, as Appl. No. 17/590,944.
Application 17/590,944 is a continuation of application No. 16/738,863, filed on Jan. 9, 2020, granted, now 11,243,219.
Application 16/738,863 is a continuation of application No. 15/909,677, filed on Mar. 1, 2018, granted, now 10,534,006, issued on Jan. 14, 2020.
Application 15/909,677 is a continuation of application No. 14/849,348, filed on Sep. 9, 2015, granted, now 9,910,053, issued on Mar. 6, 2018.
Claims priority of provisional application 62/048,183, filed on Sep. 9, 2014.
Prior Publication US 2022/0155326 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/86 (2006.01); G01N 33/49 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01)
CPC G01N 33/86 (2013.01) [B01L 3/502715 (2013.01); B01L 9/527 (2013.01); G01N 33/4905 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/1827 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A test microchip for measuring clotting time in a blood or plasma sample, the test microchip comprising:
an inlet for the blood or plasma sample, the inlet communicating with one or more microchannels having a length between tens of microns and millimeters, each microchannel comprising one or more test chambers, each microchannel having a defined volume between nanoliters and milliliters and configured to draw the blood or plasma sample into the one or more test chambers by passive capillary action, the one or more microchannels communicating with an outlet and each of the one or more microchannels comprising at least one anionically charged surface configured to activate clotting of the blood or plasma sample upon entry of the blood or plasma sample into the one or more microchannels or test chamber; wherein the anionically charged surface does not include chemical agents activating clotting, and two or more integrated electrodes which allow changes in electrical impedance in the blood or plasma to be measured to determine clot formation in the blood or plasma sample.