US 11,852,629 B2
Method and device for determining autophagic flux
Benjamin Loos, Stellenbosch (ZA); Jan Hendrik Servaas Hofmeyr, Stellenbosch (ZA); Willem Jacobus Perold, Stellenbosch (ZA); and Andre Du Toit, Stellenbosch (ZA)
Assigned to Stellenbosch University, Stellenbosch (ZA)
Appl. No. 17/254,489
Filed by Stellenbosch University, Stellenbosch (ZA)
PCT Filed Jul. 1, 2019, PCT No. PCT/IB2019/055604
§ 371(c)(1), (2) Date Dec. 21, 2020,
PCT Pub. No. WO2020/003286, PCT Pub. Date Jan. 2, 2020.
Claims priority of application No. 2018/04374 (ZA), filed on Jun. 29, 2018.
Prior Publication US 2021/0116446 A1, Apr. 22, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/5438 (2013.01) [G01N 33/6845 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A method for determining autophagic flux, the method comprising the steps of:
determining, in a biological sample from a subject, the level of at least two proteins which are overexpressed or underexpressed with a change in autophagic flux, wherein the proteins are selected from the group consisting of Serine/threonine-protein kinase DCLK1, Serine-tRNA ligase cytoplasmic (SARS), Sequestosome-1, Acetoacetyl-CoA synthetase, V-type proton ATPase catalytic subunit A, Glyceraldehyde-3-phosphate dehydrogenase, Histone-binding protein RBBP7, DnaJ homolog subfamily B member 4, Microtubule-associated proteins 1A/1B light chain 3B, Cysteine protease ATG4B, Lysosome-associated membrane glycoprotein 2, Tubulin beta-2A chain, Mammalian target of rapamycin and Phospho-mammalian target of rapamycin;
determining, in the biological sample, the level of a reference protein which is not differently expressed with a change in autophagy;
comparing the level of each of the at least two proteins to the level of each of the other proteins which are overexpressed or underexpressed with a change in autophagic flux and comparing these proteins to the level of a reference protein; and
obtaining a flux value for the sample.