CPC G01N 33/5438 (2013.01) [G01N 33/6845 (2013.01)] | 12 Claims |
1. A method for determining autophagic flux, the method comprising the steps of:
determining, in a biological sample from a subject, the level of at least two proteins which are overexpressed or underexpressed with a change in autophagic flux, wherein the proteins are selected from the group consisting of Serine/threonine-protein kinase DCLK1, Serine-tRNA ligase cytoplasmic (SARS), Sequestosome-1, Acetoacetyl-CoA synthetase, V-type proton ATPase catalytic subunit A, Glyceraldehyde-3-phosphate dehydrogenase, Histone-binding protein RBBP7, DnaJ homolog subfamily B member 4, Microtubule-associated proteins 1A/1B light chain 3B, Cysteine protease ATG4B, Lysosome-associated membrane glycoprotein 2, Tubulin beta-2A chain, Mammalian target of rapamycin and Phospho-mammalian target of rapamycin;
determining, in the biological sample, the level of a reference protein which is not differently expressed with a change in autophagy;
comparing the level of each of the at least two proteins to the level of each of the other proteins which are overexpressed or underexpressed with a change in autophagic flux and comparing these proteins to the level of a reference protein; and
obtaining a flux value for the sample.
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