CPC G01B 11/14 (2013.01) [G01N 21/64 (2013.01); G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G02B 21/0056 (2013.01); G02B 21/0072 (2013.01); G02B 21/0076 (2013.01); G02B 21/16 (2013.01); G01B 11/25 (2013.01); G01N 2201/06113 (2013.01); G01N 2201/12 (2013.01)] | 4 Claims |
1. A method for measuring a position of a fluorophore, the method comprising:
configuring a set of compact light distributions, the set having at least one member, each light distribution characterized by a center, so that there is substantially zero intensity at the center of the set of compact light distributions;
moving the set of compact light distributions in relation to a set of hypothesized positions of the fluorophore;
detecting, in a plurality of locations corresponding to the hypothesized set of positions, a set of images; and
estimating the position of the fluorophore, by determining from the set of images a set of parameters describing the position of the fluorophore using an inverse problem method.
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