CPC C12N 9/1252 (2013.01) [C12N 15/63 (2013.01); C12Q 1/6874 (2013.01); C12Y 207/07007 (2013.01)] | 17 Claims |
1. A method of sequencing a nucleic acid sequence comprising:
a) hybridizing a nucleic acid template with a primer to form a primer-template hybridization complex;
b) contacting the primer-template hybridization complex with a DNA polymerase and nucleotides, wherein each of the nucleotides comprises a detectable label;
c) subjecting the primer-template hybridization complex to conditions which enable the polymerase to incorporate a nucleotide into the primer-template hybridization complex to form a modified primer-template hybridization complex;
d) detecting the detectable label; thereby sequencing a nucleic acid sequence;
wherein the DNA polymerase comprises an amino acid sequence that is at least 80% identical to a continuous 500 amino acid sequence within SEQ ID NO: 1; comprising the following amino acids:
an alanine at amino acid position 409 or at an amino acid position functionally equivalent to amino acid position 409;
a glycine at amino acid position 410 or at an amino acid position functionally equivalent to amino acid position 410;
a proline at amino acid position 411 or at an amino acid position functionally equivalent to amino acid position 411.
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