US 11,851,684 B2
Expression cassette
Masahiro Murakami, Tsuruoka (JP)
Assigned to Spiber Inc., Yamagata (JP)
Appl. No. 16/649,804
Filed by Spiber Inc., Yamagata (JP)
PCT Filed Sep. 28, 2018, PCT No. PCT/JP2018/036259
§ 371(c)(1), (2) Date Mar. 23, 2020,
PCT Pub. No. WO2019/065968, PCT Pub. Date Apr. 4, 2019.
Claims priority of application No. 2017-191063 (JP), filed on Sep. 29, 2017.
Prior Publication US 2020/0308552 A1, Oct. 1, 2020
Int. Cl. C12N 5/10 (2006.01); C12N 15/11 (2006.01); C12P 21/02 (2006.01)
CPC C12N 5/10 (2013.01) [C12N 15/111 (2013.01); C12P 21/02 (2013.01)] 16 Claims
 
1. An expression cassette comprising, in a 5′ to 3′ direction of a sense strand, a promoter, and a first nucleic acid, a terminator and a second nucleic acid operably linked to the promoter, wherein the first nucleic acid and the second nucleic acid each comprises at least one gene, and the first nucleic acid and second nucleic acid are expressed under control of same said promoter, wherein an expression level of the gene comprised in the second nucleic acid is 20% or less of an expression level of the gene comprised in the first nucleic acid.