CPC C12N 5/0693 (2013.01) [C12Q 1/6806 (2013.01); G01N 33/5044 (2013.01)] | 25 Claims |
13. A method, comprising:
(a) providing a plurality of cells comprising a cell comprising a plurality of analytes;
(b) contacting the plurality of cells with a targeting agent coupled to a barcoding activator, to provide the cell coupled to the barcoding activator via the targeting agent wherein the barcoding activator comprises a nuclease enzyme; and
(c) partitioning the cell and a plurality of nucleic acid barcode molecules in a partition of a plurality of partitions, wherein in the partition, the nuclease enzyme of the barcoding activator cleaves a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules to facilitate barcoding of an analyte of the plurality of analytes with the nucleic acid barcode molecule.
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