US 11,851,683 B1
Methods and systems for selective analysis of cellular samples
Arundhati Shamoni Maheshwari, Oakland, CA (US); and Marlon Stoeckius, Stockholm (SE)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X GENOMICS, INC., Pleasanton, CA (US)
Filed on Feb. 12, 2020, as Appl. No. 16/789,273.
Claims priority of provisional application 62/804,575, filed on Feb. 12, 2019.
Int. Cl. G01N 33/50 (2006.01); C12Q 1/6806 (2018.01); C12N 5/09 (2010.01)
CPC C12N 5/0693 (2013.01) [C12Q 1/6806 (2013.01); G01N 33/5044 (2013.01)] 25 Claims
OG exemplary drawing
 
13. A method, comprising:
(a) providing a plurality of cells comprising a cell comprising a plurality of analytes;
(b) contacting the plurality of cells with a targeting agent coupled to a barcoding activator, to provide the cell coupled to the barcoding activator via the targeting agent wherein the barcoding activator comprises a nuclease enzyme; and
(c) partitioning the cell and a plurality of nucleic acid barcode molecules in a partition of a plurality of partitions, wherein in the partition, the nuclease enzyme of the barcoding activator cleaves a nucleic acid barcode molecule of the plurality of nucleic acid barcode molecules to facilitate barcoding of an analyte of the plurality of analytes with the nucleic acid barcode molecule.