US 11,851,671 B2
Programmable assembly of virus composites for receptor-targeted gene delivery
Daniel Schmidt, Minneapolis, MN (US); Wendy Gordon, St. Paul, MN (US); and Alina Catherine Zdechlik, Minnetonka, MN (US)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US)
Appl. No. 16/758,602
Filed by Regents of the University of Minnesota, Minneapolis, MN (US)
PCT Filed Oct. 23, 2018, PCT No. PCT/US2018/057119
§ 371(c)(1), (2) Date Apr. 23, 2020,
PCT Pub. No. WO2019/084015, PCT Pub. Date May 2, 2019.
Claims priority of provisional application 62/575,722, filed on Oct. 23, 2017.
Prior Publication US 2021/0180082 A1, Jun. 17, 2021
Int. Cl. C12N 15/86 (2006.01); C07K 14/47 (2006.01); C07K 16/28 (2006.01); A61K 48/00 (2006.01); C12N 15/864 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 48/0008 (2013.01); C07K 14/47 (2013.01); C07K 16/28 (2013.01); C12N 15/8645 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A helper-free population of infectious recombinant adeno-associated virus (AAV) with altered tropism, comprising: infectious recombinant AAV comprising a modified AAV capsid having an insertion that includes one or more single stranded nucleic acid binding domains and a modified heparin binding domain, and a viral genome, wherein the insertion is bound to a molecule capable of providing the altered tropism which is altered relative to a corresponding AAV without the modification in the viral capsid, wherein the modified heparin binding domain has decreased binding to heparin relative to a corresponding AAV capsid having an unmodified heparin binding domain, wherein the one or more single stranded nucleic acid binding domains are HUH endonuclease domains.