US 11,851,664 B2
Methods for producing biochemicals using enzyme genes derived from a strain of Brevundimonas, and compositions made thereby
Chia-Han Song, Palo Alto, CA (US); William J. Coleman, Redwood City, CA (US); and Brian Sefton, Sunnyvale, CA (US)
Assigned to OAKBIO, INC., Sunnyvale, CA (US)
Filed by Oakbio, Inc., Sunnyvale, CA (US)
Filed on Aug. 5, 2021, as Appl. No. 17/395,421.
Claims priority of provisional application 63/130,569, filed on Dec. 24, 2020.
Prior Publication US 2022/0204979 A1, Jun. 30, 2022
Int. Cl. C12N 15/74 (2006.01); C12N 9/02 (2006.01)
CPC C12N 15/74 (2013.01) [C12N 9/0083 (2013.01); C12N 2800/101 (2013.01)] 9 Claims
 
1. An expression construct comprising:
a nucleic acid sequence for a crtW carotenoid ketolase gene from Brevundimonas strain OB307 that encodes the amino acid sequence of SEQ ID NO: 2, the expression construct adapted to produce carotenoids in a heterologous biological host cell.