	US 11,851,662 B2
	Methods and compositions for promoting non-natural amino acid-containing protein production
Sigeng Chen, San Diego, CA (US); Yingchun Lu, San Diego, CA (US); and Feng Tian, San Diego, CA (US)
Assigned to Ambrx, Inc., La Jolla, CA (US)
Appl. No. 16/958,152
Filed by Ambrx, Inc., La Jolla, CA (US)
PCT Filed Jun. 2, 2018, PCT No. PCT/US2018/035764 § 371(c)(1), (2) Date Jun. 26, 2020, PCT Pub. No. WO2018/223108, PCT Pub. Date Dec. 6, 2018.
Claims priority of provisional application 62/514,754, filed on Jun. 2, 2017.
Prior Publication US 2021/0017527 A1, Jan. 21, 2021
Int. Cl. C12N 15/67 (2006.01); C12N 5/10 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/67 (2013.01) [C12N 5/10 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05); C12N 2501/48 (2013.01); C12N 2510/02 (2013.01)]

24 Claims

1. A method of generating a cell line for incorporating a non-natural amino acid into a protein, the method comprising:

inactivating one or more target site(s) or region(s) in a cell, wherein the one or more target site(s) or region(s) is involved in an apoptotic pathway, and

introducing a nucleic acid selected from the group consisting of SEQ ID NOs: 1 to 6 into the cell, wherein the nucleic acid is capable of inactivating the one or more target site(s) or region(s) in the cell;

wherein the cell expresses a selector codon-containing gene of interest, and wherein the cell comprises an orthogonal aminoacyl tRNA synthetase and an orthogonal suppressor tRNA.