US 11,851,661 B2
Polypeptide expression systems
Isidro Hotzel, Brisbane, CA (US); and Yonglei Shang, Millbrae, CA (US)
Assigned to Genentech, Inc., South San Francisco, CA (US)
Filed by Genentech, Inc., South San Francisco, CA (US)
Filed on Apr. 17, 2019, as Appl. No. 16/387,054.
Application 16/387,054 is a continuation of application No. 14/790,818, filed on Jul. 2, 2015, granted, now 10,308,944.
Claims priority of provisional application 62/080,698, filed on Nov. 17, 2014.
Claims priority of provisional application 62/020,838, filed on Jul. 3, 2014.
Prior Publication US 2019/0256859 A1, Aug. 22, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/63 (2006.01); C12P 21/00 (2006.01); C07K 16/00 (2006.01)
CPC C12N 15/63 (2013.01) [C07K 16/005 (2013.01); C12P 21/00 (2013.01); C07K 2317/10 (2013.01); C07K 2317/52 (2013.01); C07K 2317/55 (2013.01); C07K 2317/60 (2013.01); C07K 2317/62 (2013.01); C07K 2317/64 (2013.01)] 57 Claims
 
1. A polypeptide expression system comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein:
(a) the first nucleic acid molecule comprises a first expression cassette comprising the following components: (i) a first eukaryotic promoter (P1Euk1), (ii) a first polypeptide-encoding sequence (PES11), (iii) a first 5′ splice site (5′ss11), and (iv) a hybridizing sequence (HS1), wherein the components are operably linked to each other in a 5′-to-3′ direction as P1Euk1-PES11-5′ss11-HS1, wherein the HS1 and is a gene encoding all or a poriton of a pIII coat protien of bacteriophage M13, and wherein the HS1 comprises a mutated polyadenlation site; and
(b) the second nucleic acid molecule comprises the following components: (i) a eukaryotic promoter (P2Euk), (ii) a hybridizing sequence capable of hybridizing to HS1 (HS2), (iii) a 3′ splice site (3′ss2), (iv) a second polypeptide-encoding sequence (PES2), and (v) a polyadenylation site (pA2), wherein the components are operably linked to each other in a 5′-to-3′ direction as P2Euk-HS2-3′ss2-PES2-pA2.