CPC C12N 15/63 (2013.01) [C07K 16/005 (2013.01); C12P 21/00 (2013.01); C07K 2317/10 (2013.01); C07K 2317/52 (2013.01); C07K 2317/55 (2013.01); C07K 2317/60 (2013.01); C07K 2317/62 (2013.01); C07K 2317/64 (2013.01)] | 57 Claims |
1. A polypeptide expression system comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein:
(a) the first nucleic acid molecule comprises a first expression cassette comprising the following components: (i) a first eukaryotic promoter (P1Euk1), (ii) a first polypeptide-encoding sequence (PES11), (iii) a first 5′ splice site (5′ss11), and (iv) a hybridizing sequence (HS1), wherein the components are operably linked to each other in a 5′-to-3′ direction as P1Euk1-PES11-5′ss11-HS1, wherein the HS1 and is a gene encoding all or a poriton of a pIII coat protien of bacteriophage M13, and wherein the HS1 comprises a mutated polyadenlation site; and
(b) the second nucleic acid molecule comprises the following components: (i) a eukaryotic promoter (P2Euk), (ii) a hybridizing sequence capable of hybridizing to HS1 (HS2), (iii) a 3′ splice site (3′ss2), (iv) a second polypeptide-encoding sequence (PES2), and (v) a polyadenylation site (pA2), wherein the components are operably linked to each other in a 5′-to-3′ direction as P2Euk-HS2-3′ss2-PES2-pA2.
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