	US 11,851,630 B2
	Polypeptides having phospholipase C activity and polynucleotides encoding same
Dorotea Raventos Segura, Rungsted (DK); Robert Piotr Olinski, Vaerlose (DK); Kim Borch, Birkerod (DK); Allan Noergaard, Lyngby (DK); Jesper Brask, Vaerlose (DK); Rune Falkenberg Haller, Roskilde (DK); and Marianne Linde Damstrup, Gentofte (DK)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on Feb. 17, 2021, as Appl. No. 17/177,349.
Application 17/177,349 is a division of application No. 16/423,748, filed on May 28, 2019, granted, now 10,954,469.
Application 16/423,748 is a division of application No. 15/126,098, granted, now 10,351,795, issued on Jul. 16, 2019, previously published as PCT/EP2015/055856, filed on Mar. 19, 2015.
Claims priority of application No. 14160698 (EP), filed on Mar. 19, 2014.
Prior Publication US 2021/0171860 A1, Jun. 10, 2021
Int. Cl. C12N 9/16 (2006.01); C11B 3/00 (2006.01); C12P 7/6445 (2022.01); C12N 9/20 (2006.01)

CPC C11B 3/003 (2013.01) [C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12P 7/6445 (2013.01); C12Y 301/04003 (2013.01); C12Y 301/04011 (2013.01)]

12 Claims

1. A method for reducing the content of phospholipids in an oil composition, the method comprising

a) contacting an oil composition containing a quantity of phospholipids with a phosphatidylinositol phospholipase C and a phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C polypeptide (“a PC and PE-specific phospholipase C”) under conditions sufficient to hydrolyze the phospholipids to diglyceride and phosphate ester, wherein

i) the phosphatidylinositol phospholipase C has at least 90% sequence identity to amino acid residues 26-322 of SEQ ID NO: 2 or amino acid residues 1-298 of SEQ ID NO: 3, and

(ii) the PC and PE-specific phospholipase C has at least 90% sequence identity to amino acid residues 34-278 of SEQ ID NO: 19 or amino acid residues 1-246 of SEQ ID NO: 20; and

b) separating the phosphate ester from the oil composition.