	US 12,503,722 B2
	Enzymatic DNA repair
Robert N. Grass, Zurich (CH); Alexander Xavier Christof Kohll, Zurich (CH); and Weida Chen, Zurich (CH)
Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed by MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed on Aug. 31, 2023, as Appl. No. 18/459,183.
Application 18/459,183 is a division of application No. 16/152,808, filed on Oct. 5, 2018, granted, now 11,781,169.
Prior Publication US 2024/0011076 A1, Jan. 11, 2024
Int. Cl. C12Q 1/6806 (2018.01); G06N 3/123 (2023.01)

CPC C12Q 1/6806 (2013.01) [G06N 3/123 (2013.01)]

20 Claims

1. A method of storing digital information in deoxyribose nucleic acid (DNA) comprising:

encoding the digital information as a sequence of nucleotides;

synthesizing a synthetic DNA molecule having the sequence of nucleotides;

storing the synthetic DNA molecule;

determining that a quantity of PCR-amplifiable DNA that is stored is less than a threshold level, wherein the threshold level is based on a change in the quantity of PCR-amplifiable DNA before and after storage as measured by quantitative PCR;

contacting the synthetic DNA molecule with a repair mixture comprising at least one of a DNA polymerase, a DNA ligase, or an endonuclease;

sequencing the synthetic DNA molecule following the contacting with the repair mixture to generate sequence data; and

decoding the sequence data to obtain regenerated digital information.