US 11,841,491 B2
Observation vessel, sample preparation method, and observation method
Yoshihiro Shimada, Tokyo (JP); Chihiro Hiraga, Tokyo (JP); Katsunori Ogo, Tokyo (JP); Daisuke Nishiwaki, Tokyo (JP); and Takashi Sugiyama, Tokyo (JP)
Assigned to Evident Corporation, Nagano (JP)
Filed by Evident Corporation, Nagano (JP)
Filed on May 24, 2021, as Appl. No. 17/327,891.
Claims priority of application No. 2020-091643 (JP), filed on May 26, 2020.
Prior Publication US 2021/0373312 A1, Dec. 2, 2021
Int. Cl. G02B 21/06 (2006.01); G02B 21/34 (2006.01); G02B 21/00 (2006.01)
CPC G02B 21/06 (2013.01) [G02B 21/0076 (2013.01); G02B 21/34 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An observation vessel comprising:
a holding portion configured to hold an observation object; and
an accommodation portion that is at least partially formed of a bottom having a transparent three-dimensional curved surface and is configured to position the holding portion,
wherein the holding portion is configured to hold the observation object at a set position separated from the three-dimensional curved surface toward a center of curvature of the three-dimensional curved surface inside the accommodation portion in a first state of being positioned at a first position by the accommodation portion, and
wherein the observation vessel is configured to guide light from the observation object held at the set position into an objective lens positioned below the bottom of the accommodation portion via the three-dimensional curved surface,
wherein the three-dimensional curved surface and a positional relationship between the set position and the three-dimensional curved surface suppresses spherical aberration caused by refractive index mismatching between the observation vessel and a medium in the observation vessel or between the medium in the observation vessel and an immersion liquid between the observation vessel and the objective lens.