US 11,841,358 B2
Methods and systems for determining platelet concentration
Huan Qi, Shenzhen (CN); Bo Ye, Shenzhen (CN); Wenbo Zheng, Shenzhen (CN); Changsong Hu, Shenzhen (CN); and Qi Yu, Shenzhen (CN)
Assigned to Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen (CN)
Filed by Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen (CN)
Filed on Oct. 20, 2020, as Appl. No. 17/075,603.
Application 17/075,603 is a continuation of application No. PCT/CN2019/084687, filed on Apr. 26, 2019.
Claims priority of application No. PCT/CN2018/085197 (WO), filed on Apr. 28, 2018.
Prior Publication US 2021/0033593 A1, Feb. 4, 2021
Int. Cl. C12Q 1/00 (2006.01); G01N 33/49 (2006.01); G16H 40/67 (2018.01); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G01N 15/06 (2006.01); G01N 33/487 (2006.01); G01N 15/00 (2006.01)
CPC G01N 33/49 (2013.01) [G01N 15/0656 (2013.01); G01N 33/48707 (2013.01); G16H 10/40 (2018.01); G16H 15/00 (2018.01); G16H 40/67 (2018.01); G01N 2015/0084 (2013.01); G01N 2015/0693 (2013.01)] 20 Claims
 
1. A method for determining a platelet concentration in a blood sample comprising:
mixing a first aliquot of the blood sample with a diluent to form a first suspension;
mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells to form a second suspension;
measuring DC impedance signals of the first suspension passing through an aperture;
measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell;
analyzing the DC impedance signals of the first suspension to obtain a first platelet distribution;
analyzing the light scatter signals and the fluorescent signals of the second suspension to differentiate platelets from white blood cells and/or nucleated red blood cells and to obtain a second platelet distribution; and
determining the platelet concentration of the blood sample using the first and second platelet distributions.