US 11,840,713 B2
Glucoamylase variants and polynucleotides encoding same
Esben Peter Friis, Herlev (DK); Leonardo De Maria, Frederiksberg (DK); Jesper Vind, Vaerloese (DK); Thomas A. Poulsen, Ballerup (DK); Allan Svendsen, Hoersholm (DK); Steffen Danielsen, Copenhagen Oe (DK); Rolf T. Lenhard, Lyngby (DK); Henrik Friis-Madsen, Ballerup (DK); and Lars K. Skov, Ballerup (DK)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by NOVOZYMES A/S, Bagsvaerd (DK)
Filed on Nov. 10, 2020, as Appl. No. 17/093,911.
Application 17/093,911 is a division of application No. 16/135,407, filed on Sep. 19, 2018, granted, now 10,865,399.
Application 15/677,604 is a division of application No. 15/362,915, filed on Nov. 29, 2016, granted, now 9,765,318, issued on Sep. 19, 2017.
Application 15/362,915 is a division of application No. 15/139,050, filed on Apr. 26, 2016, granted, now 9,540,624, issued on Jan. 10, 2017.
Application 15/139,050 is a division of application No. 14/351,469, granted, now 9,353,362, issued on May 31, 2016, previously published as PCT/EP2012/070127, filed on Oct. 11, 2012.
Application 16/135,407 is a continuation of application No. 15/677,604, filed on Aug. 15, 2017, granted, now 10,113,158, issued on Oct. 30, 2018.
Claims priority of provisional application 61/545,628, filed on Oct. 11, 2011.
Prior Publication US 2021/0062172 A1, Mar. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/04 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 9/34 (2006.01); C12P 19/20 (2006.01); C12P 5/02 (2006.01); C12P 7/06 (2006.01); C12P 7/54 (2006.01); C12P 13/20 (2006.01); C12P 37/00 (2006.01); C12N 9/30 (2006.01); C12P 5/00 (2006.01); C12P 7/46 (2006.01); C12P 7/48 (2006.01); C12P 7/56 (2006.01)
CPC C12N 9/2428 (2013.01) [C12N 9/242 (2013.01); C12P 5/002 (2013.01); C12P 5/02 (2013.01); C12P 7/06 (2013.01); C12P 7/46 (2013.01); C12P 7/48 (2013.01); C12P 7/54 (2013.01); C12P 7/56 (2013.01); C12P 13/20 (2013.01); C12P 19/20 (2013.01); C12P 37/00 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); Y02E 50/10 (2013.01)] 17 Claims
 
1. An isolated polynucleotide encoding a polypeptide, wherein the polypeptide is a glucoamylase variant comprising a substitution or deletion at one or more positions corresponding to positions 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 18, 26, 31, 33, 34, 65, 72, 74, 79, 80, 103, 105, 112, 161, 172, 218, 220, 221, 245, 253, 255, 279, 325, 327, 359, 364, 370, 375, 377, 405, 445, 447, 460, 463, 465, 468, 477, 501, 502, 504, 516, 524, 526, 563, 564, 568, 571 of the polypeptide of SEQ ID NO: 3, wherein the variant has glucoamylase activity and at least 70 percent sequence identity to the polypeptide of SEQ ID NO: 3.