US 11,840,697 B2
Haploid induction compositions and methods for use therefor
Timothy Kelliher, Research Triangle Park, NC (US); Satya Chintamanani, Slater, IA (US); Brent Delzer, Janesville, WI (US); Michael L Nuccio, Durham, NC (US); Robert Arthur Dietrich, Research Triangle Park, NC (US); Suresh Babu Kadaru, Hydrabad (IN); Todd Lee Warner, Stanton, MN (US); and William Paul Bullock, Slater, IA (US)
Assigned to Syngenta Participations AG, Basel (CH)
Filed by Syngenta Participations AG, Basel (CH)
Filed on Feb. 12, 2021, as Appl. No. 17/174,515.
Application 17/174,515 is a division of application No. 16/218,529, filed on Dec. 13, 2018, granted, now 10,954,523.
Application 16/218,529 is a division of application No. 15/586,649, filed on May 4, 2017, granted, now 10,190,125.
Application 15/586,649 is a division of application No. 14/212,504, filed on Mar. 14, 2014, granted, now 9,677,082, issued on Jun. 13, 2017.
Claims priority of provisional application 61/852,428, filed on Mar. 15, 2013.
Prior Publication US 2021/0163971 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C12Q 1/6895 (2018.01); C12N 9/18 (2006.01); A01H 1/04 (2006.01); A01H 1/08 (2006.01); C12Q 1/686 (2018.01)
CPC C12N 15/8216 (2013.01) [A01H 1/045 (2021.01); A01H 1/08 (2013.01); C12N 9/18 (2013.01); C12N 15/8218 (2013.01); C12N 15/8261 (2013.01); C12N 15/8287 (2013.01); C12Q 1/6895 (2013.01); C12Q 1/686 (2013.01); Y02A 40/146 (2018.01)] 2 Claims
 
1. A method of creating a new haploid inducer maize plant with a silenced patatin-like phospholipase 2A, comprising transcribing a polynucleotide sequence that silences the patatin-like phospholipase 2A in maize, wherein said polynucleotide sequence comprises a first sequence selected from the group consisting of:
a) a polynucleotide sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 34 or the complement thereof;
b) a functional fragment comprising at least 22 contiguous bases of SEQ ID NO: 34 or the complement thereof; and
c) a polynucleotide sequence having at least 95% sequence identity as determined using the BLASTN alignment tool to the nucleic acid sequence set forth in SEQ ID NO: 34 or the complement thereof;
and a second sequence that is the complement of the first sequence, wherein the polynucleotide sequence expresses a double-stranded ribonucleotide sequence which silences the patatin-like phospholipase 2A when contacted with a maize plant and thus creates a new haploid inducer maize plant.