	US 11,840,556 B2
	Modified nanopores, compositions comprising the same, and uses thereof
Lakmal Jayasinghe, Oxford (GB); Elizabeth Jayne Wallace, Oxford (GB); and Pratik Raj Singh, Oxford (GB)
Assigned to Oxford Nanopore Technologies PLC, Oxford (GB)
Appl. No. 16/484,798
Filed by Oxford Nanopore Technologies PLC, Oxford (GB)
PCT Filed Feb. 12, 2018, PCT No. PCT/GB2018/050379 § 371(c)(1), (2) Date Aug. 8, 2019, PCT Pub. No. WO2018/146491, PCT Pub. Date Aug. 16, 2018.
Claims priority of provisional application 62/457,483, filed on Feb. 10, 2017.
Prior Publication US 2020/0010511 A1, Jan. 9, 2020
Int. Cl. A61K 39/02 (2006.01); C07K 14/195 (2006.01); C12Q 1/6869 (2018.01); B82Y 30/00 (2011.01)

CPC C07K 14/195 (2013.01) [C12Q 1/6869 (2013.01); B82Y 30/00 (2013.01)]

17 Claims

1. A nanopore sequencing apparatus comprising a chamber housing an aqueous solution having disposed therein a membrane comprising a modified secretin nanopore disposed in the membrane, the modified secretin nanopore comprising a lumenal surface defining a lumen that extends through the membrane between a cis-opening and a trans-opening, wherein the lumenal surface comprises one or more amino acid modifications relative to a wild-type secretin nanopore.