US 11,839,878 B2
System and method for sex sorting of mosquitoes
Stephen L. Dobson, Lexington, KY (US); James William Mains, Morehead, KY (US); Jim L Peyton, Richmond, KY (US); and Hakim Sultanov, Lexington, KY (US)
Assigned to MosquitoMate, Inc., Lexington, KY (US)
Filed by MosquitoMate, Inc., Lexington, KY (US)
Filed on Nov. 30, 2022, as Appl. No. 18/060,087.
Application 18/060,087 is a division of application No. 16/546,581, filed on Aug. 21, 2019, granted, now 11,541,395.
Claims priority of provisional application 62/720,386, filed on Aug. 21, 2018.
Prior Publication US 2023/0104229 A1, Apr. 6, 2023
Int. Cl. B01L 3/00 (2006.01); A01M 1/06 (2006.01); G06V 20/10 (2022.01); H04N 23/54 (2023.01)
CPC B01L 3/50853 (2013.01) [A01M 1/06 (2013.01); G06V 20/10 (2022.01); H04N 23/54 (2023.01); B01L 2300/04 (2013.01); B01L 2300/0829 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for sex sorting of mosquitoes, comprising the steps of:
providing a plate that includes multiple wells, with each of the multiple wells configured to receive a mosquito;
filling each of the multiple wells with a predetermined volume of water;
placing a mosquito in each of the multiple wells;
providing an imaging subsystem, including (a) camera and (b) a computer including a processor;
capturing an image of each mosquito in each of the multiple wells via the camera of the imaging subsystem according to instructions programmed into and executed by the processor;
transmitting each image to the computer of the imaging subsystem;
using the processor of the computer to analyze each image to determine if each mosquito is male or female according to instructions programmed into and executed by the processor; and
outputting the determination of whether each mosquito is male or female according to instructions programmed into and executed by the processor.
 
5. A method for sex sorting of mosquitoes, comprising the steps of:
providing a plate that includes multiple wells, with each of the multiple wells configured to receive a mosquito;
placing a mosquito in each of the multiple wells; and
using an imaging subsystem to (i) capture an image of each mosquito in each of the multiple wells, and (ii) analyze each image to determine if each mosquito is male or female;
wherein the imaging subsystem includes:
a camera for capturing each image of each mosquito, and
a computer operably connected to the camera and including a processor programmed to execute instructions to (i) capture the image of each mosquito in each of the multiple wells, and (ii) analyze each image to determine if each mosquito is male or female.
 
12. A method for sex sorting of mosquitoes, comprising the steps of:
providing a plate that includes multiple wells, with each of the multiple wells configured to receive a mosquito;
filling each of the multiple wells with a predetermined volume of water;
placing a mosquito in each of the multiple wells; and
providing an imaging subsystem, including (a) a camera and (b) a computer including a processor, wherein the processor is programmed to execute instructions for (i) capturing an image of each mosquito in each of the multiple wells, (ii) analyzing each image to determine if each mosquito is male or female, and (iii) outputting the determination of whether each mosquito is male or female.
 
15. A method for sex sorting of mosquitoes, comprising the steps of:
providing a plate that includes multiple wells, with each of the multiple wells configured to receive a mosquito;
filling each of the multiple wells with a predetermined volume of water;
placing a mosquito in each of the multiple wells;
providing an imaging subsystem, including (a) camera and (b) a computer including a processor;
capturing one or more images of the plate via the camera of the imaging subsystem according to instructions programmed into and executed by the processor;
transmitting each image to the computer of the imaging subsystem;
using the processor of the computer to analyze each image to identify one or more mosquitos in the image and to determine whether each of the one or more mosquitoes is male or female according to instructions programmed into and executed by the processor; and
outputting the determination of whether each of the one or more mosquitoes is male or female according to instructions programmed into and executed by the processor.