US 11,839,876 B2
Apparatus for microfluidic flow cytometry analysis of a particulate containing fluid
Dmitry Kashanin, Dublin (IE); Igor Shvets, Dublin (IE); and Francesco Dicorato, Dublin (IE)
Assigned to Cellix Limited, Dublin (IE)
Appl. No. 16/303,929
Filed by Cellix Limited, Dublin (IE)
PCT Filed May 24, 2017, PCT No. PCT/EP2017/062574
§ 371(c)(1), (2) Date Nov. 21, 2018,
PCT Pub. No. WO2017/202932, PCT Pub. Date Nov. 30, 2017.
Claims priority of application No. 16171121 (EP), filed on May 24, 2016.
Prior Publication US 2020/0316603 A1, Oct. 8, 2020
Int. Cl. B01L 3/00 (2006.01); G01N 15/14 (2006.01)
CPC B01L 3/502776 (2013.01) [B01L 3/502761 (2013.01); G01N 15/1404 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0809 (2013.01); G01N 2015/149 (2013.01); G01N 2015/1413 (2013.01); G01N 2015/1415 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A microfluidic chip for microfluidic flow analysis of a particulate containing fluid, the microfluidic chip comprising:
at least two layers;
a microfluidic channel that is substantially orthogonal to a plane of the layers of the chip comprising a fluid inlet for receipt of a stream of particulate containing fluid; and
a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel,
wherein the at least one pair of electrodes comprise an excitation electrode coupled to an AC signal source and a detection electrode configured to detect AC impedance changes in the microfluidic channel between the electrodes resulting from particles passing between the electrodes in the microfluidic channel,
wherein the microfluidic channel is a straight channel that extends through the at least two layers of the microfluidic chip, and
wherein the detection zone spans at least one of the layers of the microfluidic chip.