Topical application of CRISPR-modified bacteria to treat acne vulgaris
Tye Jensen, Telluride, CO (US); and Joseph E. Kovarik, Englewood, CO (US)
Assigned to Seed Health, Inc., Venice (CA)
Filed by Seed Health, Inc., Venice, CA (US)
Filed on Mar. 28, 2023, as Appl. No. 18/178,847.
Application 18/178,847 is a continuation in part of application No. 18/087,545, filed on Dec. 22, 2022.
Application 18/087,545 is a continuation in part of application No. 17/854,422, filed on Jun. 30, 2022, granted, now 11,672,835.
Application 17/854,422 is a continuation in part of application No. 17/848,759, filed on Jun. 24, 2022, granted, now 11,642,382.
Application 17/848,759 is a continuation in part of application No. 17/835,204, filed on Jun. 8, 2022, granted, now 11,529,379, issued on Dec. 20, 2022.
Application 17/835,204 is a continuation in part of application No. 17/567,295, filed on Jan. 3, 2022.
Application 17/567,295 is a continuation in part of application No. 17/337,600, filed on Jun. 3, 2021, granted, now 11,213,552, issued on Jan. 4, 2022.
Application 17/337,600 is a continuation in part of application No. 17/027,953, filed on Sep. 22, 2020, granted, now 11,026,982, issued on Jun. 8, 2021.
Application 17/027,953 is a continuation in part of application No. 16/917,096, filed on Jun. 30, 2020, granted, now 10,940,169, issued on Mar. 9, 2021.
Application 16/917,096 is a continuation in part of application No. 16/782,364, filed on Feb. 5, 2020, granted, now 10,835,560, issued on Nov. 17, 2020.
Application 16/782,364 is a continuation in part of application No. 16/423,375, filed on May 28, 2019, granted, now 10,555,976, issued on Feb. 11, 2020.
Application 16/423,375 is a continuation of application No. 16/160,336, filed on Oct. 15, 2018, granted, now 10,314,866, issued on Jun. 11, 2019.
Application 16/160,336 is a continuation of application No. 15/403,823, filed on Jan. 11, 2017, granted, now 10,111,913, issued on Oct. 30, 2018.
Application 16/160,336 is a continuation of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 16/426,346, filed on May 30, 2019, granted, now 10,716,815, issued on Jul. 21, 2020.
Application 16/426,346 is a continuation of application No. 15/639,767, filed on Jun. 30, 2017, granted, now 10,314,865, issued on Jun. 11, 2019.
Application 15/639,767 is a continuation in part of application No. 15/437,976, filed on Feb. 21, 2017, granted, now 9,730,967, issued on Aug. 15, 2017.
Application 15/437,976 is a continuation in part of application No. 15/228,454, filed on Aug. 4, 2016, granted, now 9,585,920, issued on Mar. 7, 2017.
Application 15/228,454 is a continuation in part of application No. 14/954,074, filed on Nov. 30, 2015, granted, now 9,457,077, issued on Oct. 4, 2016.
Application 15/228,454 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 16/776,861, filed on Jan. 30, 2020, granted, now 10,864,109, issued on Dec. 15, 2020.
Application 16/776,861 is a continuation of application No. 16/142,171, filed on Sep. 26, 2018, granted, now 10,548,761, issued on Feb. 4, 2020.
Application 16/142,171 is a continuation in part of application No. 15/395,419, filed on Dec. 30, 2016, granted, now 10,086,018, issued on Oct. 2, 2018.
Application 16/142,171 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 17/893,384, filed on Aug. 23, 2022.
Application 17/893,384 is a continuation in part of application No. 17/023,736, filed on Sep. 17, 2020, granted, now 11,419,903, issued on Aug. 23, 2022.
Application 17/023,736 is a continuation in part of application No. 17/011,175, filed on Sep. 3, 2020, granted, now 11,273,187, issued on Mar. 15, 2022.
Application 17/011,175 is a continuation in part of application No. 16/722,117, filed on Dec. 20, 2019, granted, now 10,842,834, issued on Nov. 24, 2020.
Application 16/722,117 is a continuation in part of application No. 16/229,252, filed on Dec. 21, 2018, granted, now 10,512,661, issued on Dec. 24, 2019.
Application 16/229,252 is a continuation in part of application No. 15/392,173, filed on Dec. 28, 2016, granted, now 10,245,288, issued on Apr. 2, 2019.
Application 16/229,252 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 17/738,771, filed on May 6, 2022.
Application 17/738,771 is a continuation in part of application No. 16/904,056, filed on Jun. 17, 2020, granted, now 11,523,934, issued on Dec. 13, 2022.
Application 16/904,056 is a continuation in part of application No. 15/983,250, filed on May 18, 2018, granted, now 10,687,975, issued on Jun. 12, 2020.
Application 15/983,250 is a continuation in part of application No. 15/384,716, filed on Dec. 20, 2016, granted, now 9,987,224, issued on Jun. 5, 2018.
Application 15/983,250 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 16/037,053, filed on Jul. 17, 2018, abandoned.
Application 16/037,053 is a continuation in part of application No. 17/543,992, filed on Dec. 7, 2021.
Application 17/543,992 is a continuation in part of application No. 16/804,361, filed on Feb. 28, 2020, granted, now 11,191,665, issued on Dec. 7, 2021.
Application 16/804,361 is a continuation in part of application No. 16/020,433, filed on Jun. 27, 2018, granted, now 10,583,033, issued on Mar. 10, 2020.
Application 16/020,433 is a continuation in part of application No. 15/342,642, filed on Nov. 3, 2016, granted, now 10,010,568, issued on Jul. 3, 2018.
Application 16/020,433 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 15/270,034, filed on Sep. 20, 2016, granted, now 9,750,802, issued on Sep. 5, 2017.
Application 15/270,034 is a continuation in part of application No. 14/954,074, filed on Nov. 30, 2015, granted, now 9,457,077, issued on Oct. 4, 2016.
Application 14/954,074 is a continuation in part of application No. 14/574,517, filed on Dec. 18, 2014, granted, now 9,408,880, issued on Aug. 9, 2016.
Application 14/954,074 is a continuation in part of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 17/836,079, filed on Jun. 9, 2022.
Application 17/836,079 is a continuation in part of application No. 16/884,772, filed on May 27, 2020, granted, now 11,357,722, issued on Jun. 14, 2022.
Application 16/884,772 is a continuation in part of application No. 16/136,950, filed on Sep. 20, 2018, granted, now 10,668,014, issued on Jun. 2, 2020.
Application 16/136,950 is a continuation of application No. 15/385,278, filed on Dec. 20, 2016, granted, now 10,085,938, issued on Dec. 2, 2018.
Application 16/136,950 is a continuation of application No. 18/178,847, filed on Mar. 28, 2023.
Application 18/178,847 is a continuation in part of application No. 14/752,192, filed on Jun. 26, 2015, granted, now 9,549,842, issued on Jan. 24, 2017.
Claims priority of provisional application 62/296,186, filed on Feb. 17, 2016.
Claims priority of provisional application 62/274,550, filed on Jan. 4, 2016.
Claims priority of provisional application 62/275,341, filed on Jan. 6, 2016.
Claims priority of provisional application 62/387,405, filed on Dec. 24, 2015.
Claims priority of provisional application 62/260,906, filed on Nov. 30, 2015.
Claims priority of provisional application 62/072,476, filed on Oct. 30, 2014.
Claims priority of provisional application 62/053,926, filed on Sep. 23, 2014.
Claims priority of provisional application 62/014,855, filed on Jun. 20, 2014.
Claims priority of provisional application 61/919,297, filed on Dec. 20, 2013.
Prior Publication US 2023/0218682 A1, Jul. 13, 2023
1. A method of reducing the likelihood of a skin condition comprising acne vulgaris that causes skin inflammation in an individual human being, said method comprising: administering a therapeutically effective amount of a bacterial formulation comprising at least one of a live bacteria selected from the group consisting of Lactobacillus reuteri, Lactobacillus johnsonii, Lactobacillus crispatus, Cutibacterium acnes, and Nitrosomonas eutropha, said bacterial formulation comprising a lotion, ointment or gel adapted to be rubbed onto a region of an individual's skin, wherein at least some bacteria in the bacterial formulation have been modified by using a using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Francisella 1(Cpf1) system to reduce the production of a virulence factor by at least one of said live bacteria in said bacterial formulation.