US 12,494,037 B2
Process for providing accurate counting of nuclei in cellular images
Rishik Kolpekwar, Round Rock, TX (US)
Filed by Rishik Kolpekwar, Round Rock, TX (US)
Filed on Sep. 14, 2023, as Appl. No. 18/368,348.
Prior Publication US 2024/0177447 A1, May 30, 2024
Int. Cl. G06V 10/56 (2022.01); G06V 20/69 (2022.01)
CPC G06V 10/56 (2022.01) [G06V 20/693 (2022.01); G06T 2207/20081 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A computer implemented method for analyzing images of cellular data, the computer implemented method comprising:
receiving a first set of image data, the first set of image data stored on a computer readable medium wherein the first set of image data comprises at least one image, wherein the at least one image comprises a set of image metadata associated with the at least one image;
applying an image processing algorithm to identify pixels associated with a first color and second color, wherein each color is associated with at least one cell, wherein the first color is associated with a first range of colors, and wherein the second color is associated with a second range of colors;
associating the identified pixels with a set of coordinates which comprise at least one of a x-axis coordinate and a y-axis coordinate;
receiving a data file comprising a plurality of status indicators, wherein each status indicator is associated with a plurality of indicator colors;
associating each pixel identified by the first color with a first status identifier to generate a first list of coordinates, and associating each pixel identified by the second color with a second status identifier to generate a second list of coordinates;
generating a set of positive indicators based on data from the first list of coordinates and a set of negative indicators based on data from the second list of coordinates;
generating a second set of image data set comprising the first image data set with sets of positive and negative indicators included over the original image data and containing the first and second lists of coordinates in the image metadata;
computing a first statistical analysis of the first and second lists of coordinates to show the percentage of cells with a first status identifier compared to the total number of cells associated with the at least one image;
computing a second statistical analysis indicating the total number of cells with the first status identifier and cells with the second status identifier across all images in the image data and the percentage of cells with a first status identifier compared to the total number of cells across all images in the image data;
generating a first user interface element displaying the first statistical analysis for at least one image in the second set of image data;
generating a second user interface element displaying the second statistical analysis for all the images in the second set of image data; and
generating a third user interface element comprising the first user interface element, the second user interface element, and at least a portion of the second set of image data, wherein the user interface elements are placed in a transferable file further being sent to a user device for display on a viewport associated with the user device.