US 12,493,033 B2
Method for detecting bi-specific antibody complex
Sotaro Naoi, Shizuoka (JP); and Meiri Kawazoe, Shizuoka (JP)
Assigned to CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 15/734,663
Filed by CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
PCT Filed Jun. 3, 2019, PCT No. PCT/JP2019/021972
§ 371(c)(1), (2) Date Dec. 3, 2020,
PCT Pub. No. WO2019/235420, PCT Pub. Date Dec. 12, 2019.
Claims priority of application No. 2018-106741 (JP), filed on Jun. 4, 2018.
Prior Publication US 2021/0231654 A1, Jul. 29, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 33/86 (2006.01)
CPC G01N 33/54393 (2013.01) [G01N 33/86 (2013.01); G01N 2333/96444 (2013.01); G01N 2333/9645 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for detecting a complex in a sample, the method comprising the steps of:
(1) allowing a first binding entity to bind to a complex by bringing the first binding entity into contact with a sample comprising the complex;
(2) allowing a second binding entity to bind to the complex bound to the first binding entity; and
(3) detecting the second binding entity bound to the complex;
wherein said complex comprises two or more components, at least any one of the KD values between the components is 1 nM or greater and at least two of the components are an antibody and an antigen recognized by the antibody, the first binding entity recognizes the antigen and is bound to a solid phase, and the method is conducted under a condition in which the time of contact between any particular complex in the sample and any particular antibody bound to the solid phase is ten seconds or less,
wherein the first and second binding entities are antibodies, antibody fragments and fusion polypeptides comprising antibodies or antibody fragments thereof.