US 12,493,017 B2
Testing method
Ping-Yen Chou, Kaohsiung (TW); and Po-Jui Su, Taichung (TW)
Assigned to Ping-Yen Chou, Kaohsiung (TW)
Filed by Ping-Yen Chou, Kaohsiung (TW)
Filed on Mar. 22, 2023, as Appl. No. 18/187,763.
Claims priority of application No. 111112958 (TW), filed on Apr. 5, 2022.
Prior Publication US 2023/0314387 A1, Oct. 5, 2023
Int. Cl. G01N 30/06 (2006.01); G01N 30/72 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/06 (2013.01) [G01N 30/72 (2013.01); G01N 2030/027 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A testing method for identifying the chemical state of metallic species in an ultra-trace concentration, comprising the steps of:
providing a liquid chromatograph and an inductively coupled plasma mass spectrometer in series, wherein the liquid chromatograph comprises a liquid chromatography column;
preparing a solution of a test substance containing a solution containing the metallic species and, continuously perfusing the solution into the liquid chromatography column, the concentration of the test substance in the solution being a concentration in the range of PPM to PPb, the metallic species are one of: metallic ions, metallic complex ions, metallic chelate molecules or metallic particles; and
measuring an eluted fraction of the solution by the ICP-MS to obtain a penetration curve plotting penetration ratio versus time, the penetration ratio value of the penetration curve for the test substance gradually increases with time and tends to be stable;
after obtaining the penetration curve of the test substance, a standard solution is perfused into the same liquid chromatography column or another liquid chromatography column, and obtaining a penetration curve of the standard substance for the penetration ratio and time, a presence of metallic species is determined by comparing differences in retention time and penetration ratio profile between the penetration curves of the test substance and the standard substance.