	US 12,492,451 B2
	Metal-binding protein and use thereof
Joseph A. Cotruvo, Jr., State College, PA (US); and Joseph A. Mattocks, University Park, PA (US)
Assigned to The Penn State Research Foundation, University Park, PA (US)
Appl. No. 17/272,624
Filed by The Penn State Research Foundation, University Park, PA (US)
PCT Filed Sep. 5, 2019, PCT No. PCT/US2019/049652 § 371(c)(1), (2) Date Mar. 1, 2021, PCT Pub. No. WO2020/051274, PCT Pub. Date Mar. 12, 2020.
Claims priority of provisional application 62/727,114, filed on Sep. 5, 2018.
Prior Publication US 2021/0189521 A1, Jun. 24, 2021
Int. Cl. C07K 14/195 (2006.01); C22B 3/22 (2006.01); C22B 3/42 (2006.01); C22B 59/00 (2006.01); C22B 60/02 (2006.01)

CPC C22B 60/02 (2013.01) [C07K 14/195 (2013.01); C22B 3/22 (2013.01); C22B 3/42 (2013.01); C22B 59/00 (2013.01); Y10S 530/81 (2013.01)]

18 Claims

1. An isolated metal-binding protein comprising (i) a sequence of SEQ ID NO:41 or (ii) a sequence having at least 80% identity to SEQ ID NO:41,

wherein the isolated metal-binding protein is immobilized on a substrate;

wherein the isolated metal-binding protein has an apparent K_dvalue for a trivalent rare earth element at least 10,000-fold smaller than its apparent K_dvalue for calcium.