	US 12,492,431 B2
	Sex identification specific DNA sequence and sex identification method of chinese mitten crab Eriocheir sinensis
Zhaoxia Cui, Ningbo (CN); Yanan Yang, Ningbo (CN); and Tianyi Feng, Ningbo (CN)
Assigned to NINGBO UNIVERSITY, Ningbo (CN)
Filed by NINGBO UNIVERSITY, Ningbo (CN)
Filed on Jun. 23, 2022, as Appl. No. 17/847,210.
Claims priority of application No. 202210077326.9 (CN), filed on Jan. 24, 2022.
Prior Publication US 2023/0257810 A1, Aug. 17, 2023
Int. Cl. C12Q 1/6879 (2018.01); C12Q 1/686 (2018.01)

CPC C12Q 1/6879 (2013.01) [C12Q 1/686 (2013.01)]

2 Claims

1. A sex identification method for Chinese mitten crab Eriocheir sinensis, wherein the sex identification method is achieved by identifying a specific DNA sequence which is shown in SEQ ID No: 1; the sex identification method comprises following steps:

S1, extracting genomic DNA of Chinese mitten crab Eriocheir sinensis;

S2, PCR amplification using the genomic DNA as template; an upstream primer for PCR amplification is as shown in SEQ ID No: 2 and a downstream primer is as shown in SEQ ID No: 3;

S3, examining amplified products by agarose electrophoresis, and those with specific amplified bands are females, otherwise they are males.