	US 12,492,424 B2
	Compositions and methods for detecting viral nucleic acids
Shengxi Chen, Chandler, AZ (US); Sidney Hecht, Phoenix, AZ (US); and Mingxuan Gao, Chongqing (CN)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed by ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US)
Filed on Jul. 8, 2021, as Appl. No. 17/370,859.
Application 17/370,859 is a continuation of application No. 16/016,086, filed on Jun. 22, 2018, abandoned.
Claims priority of provisional application 62/524,070, filed on Jun. 23, 2017.
Prior Publication US 2021/0403983 A1, Dec. 30, 2021
Int. Cl. C12Q 1/6888 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/682 (2018.01); C12Q 1/686 (2018.01); C12Q 1/70 (2006.01); B82Y 5/00 (2011.01)

CPC C12Q 1/6806 (2013.01) [C12Q 1/6818 (2013.01); C12Q 1/682 (2013.01); C12Q 1/686 (2013.01); C12Q 1/70 (2013.01); B82Y 5/00 (2013.01); C12N 2310/151 (2013.01)]

9 Claims

1. A DNA nanostructure comprising four DNA oligonucleotides, wherein the first oligonucleotide has the sequence of SEQ ID NO: 2, the second oligonucleotide has the sequence of SEQ ID NO: 3, the third oligonucleotide has the sequence of SEQ ID NO: 4, and the fourth oligonucleotide has a length of 30 to 80 nucleotides and comprises a sequence that is at least 95% complementary to a target viral nucleic acid sequence;

wherein the DNA nanostructure is a DNA tetrahedron comprising five double-stranded edges and one single-stranded edge; and

wherein the target viral nucleic acid sequence is a sequence from a virus selected from dengue virus, Ebola virus, human immunodeficiency virus (HIV), hepatitis B, hepatitis C, influenza, measles, Zika, yellow fever, West Nile fever, smallpox, Marburg viruses, human papillomavirus, Kaposi's sarcoma-associated herpesvirus, and human T-lymphotropic virus.