US 12,492,381 B2
Method for cold atmospheric plasma exosome therapy
Jerome Canady, Lakeland, FL (US); Xiaoqian Cheng, Fairfax, VA (US); Saravana Murthy, Owings Mills, MD (US); Lawan Ly, Annandale, VA (US); and Taisen Zhuang, Rockville, MD (US)
Assigned to Jerome Canady Research Institute for Advanced Biological and Technological Sciences, Takoma Park, MD (US)
Appl. No. 17/611,461
Filed by Jerome Canady Research Institute for Advanced Biological and Technological Sciences, Takoma Park, MD (US)
PCT Filed May 22, 2020, PCT No. PCT/US2020/034388
§ 371(c)(1), (2) Date Nov. 15, 2021,
PCT Pub. No. WO2020/237216, PCT Pub. Date Nov. 26, 2020.
Claims priority of provisional application 62/967,935, filed on Jan. 30, 2020.
Claims priority of provisional application 62/851,286, filed on May 22, 2019.
Prior Publication US 2022/0213443 A1, Jul. 7, 2022
Int. Cl. A61K 35/13 (2015.01); A61P 35/00 (2006.01); C12N 5/09 (2010.01); C12N 13/00 (2006.01)
CPC C12N 5/0693 (2013.01) [A61K 35/13 (2013.01); A61P 35/00 (2018.01); C12N 13/00 (2013.01); C12N 2523/00 (2013.01)] 13 Claims
 
1. A method for bioengineering exosomes for cancer treatment comprising the steps of:
isolating target tumor cells from a tissue sample;
culturing the isolated target tumor cells;
treating the cultured target tumor cells with cold atmospheric plasma to release exosomes from the target tumor cells; and
collecting the released exosomes;
wherein said target tumor cells comprise triple negative breast cancer cells.