US 12,490,724 B2
Transgenic rainbow shark
Alan Blake, Austin, TX (US); Richard Crockett, Wilton, CT (US); and Aidas Nasevicius, Tampa, FL (US)
Assigned to GloFish, LLC, Earth City, MO (US)
Filed by GloFish, LLC, Earth City, MO (US)
Filed on Apr. 12, 2024, as Appl. No. 18/633,883.
Application 18/633,883 is a continuation of application No. 16/961,195, granted, now 12,225,888, previously published as PCT/US2019/013072, filed on Jan. 10, 2019.
Claims priority of provisional application 62/615,628, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,625, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,634, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,638, filed on Jan. 10, 2018.
Prior Publication US 2024/0251766 A1, Aug. 1, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2024.01); A01K 67/0275 (2024.01); C12N 15/85 (2006.01)
CPC A01K 67/0275 (2013.01) [C12N 15/8509 (2013.01); A01K 2217/00 (2013.01); A01K 2227/40 (2013.01); A01K 2267/01 (2013.01)] 9 Claims
 
1. A transgenic shark sperm cell line whose genome comprises:
a) an exogenous nucleic acid sequence encoding an orange fluorescent protein, wherein the sperm cell line is deposited at the European Collection of Authenticated Cell Cultures (ECACC) as Accession Number 19010802;
b) an exogenous nucleic acid sequence encoding a purple fluorescent protein, wherein the sperm cell line is deposited at the ECACC as Accession Number 19010801;
c) an exogenous nucleic acid sequence encoding a blue fluorescent protein, wherein the sperm cell line is deposited at the ECACC as Accession Number 19010803; or
d) an exogenous nucleic acid sequence encoding a green fluorescent protein, wherein the sperm cell line is deposited at the ECACC as Accession Number 19010804.