US 11,835,513 B2
Acoustic separation of particles for bioprocessing
Jason O. Fiering, Boston, MA (US); and Kenneth T. Kotz, Newton, MA (US)
Assigned to The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US)
Filed by THE CHARLES STARK DRAPER LABORATORY, INC., Cambridge, MA (US)
Filed on Feb. 9, 2021, as Appl. No. 17/171,651.
Application 17/171,651 is a continuation of application No. 15/965,368, filed on Apr. 27, 2018, granted, now 10,914,723.
Claims priority of provisional application 62/492,044, filed on Apr. 28, 2017.
Prior Publication US 2021/0293781 A1, Sep. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/49 (2006.01); A61M 1/36 (2006.01); B01D 21/28 (2006.01); G01N 15/02 (2006.01); B01L 3/00 (2006.01); G01N 15/06 (2006.01); B03B 1/04 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2006.01)
CPC G01N 33/491 (2013.01) [A61M 1/3678 (2014.02); A61M 1/3693 (2013.01); B01D 21/283 (2013.01); B01L 3/502753 (2013.01); B03B 1/04 (2013.01); G01N 15/02 (2013.01); G01N 15/06 (2013.01); B01D 2221/10 (2013.01); B01L 3/502761 (2013.01); B01L 2200/0652 (2013.01); B01L 2400/0436 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/0288 (2013.01); G01N 2015/0681 (2013.01); G01N 2015/0687 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1081 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A system for microfluidic particle separation configured to separate target particles from non-target particles in a biofluid, the system comprising:
at least one microfluidic separation channel comprising at least one inlet, a first outlet, and a second outlet, a first collection channel in fluid communication with the first outlet and a second collection channel in fluid communication with the second outlet;
a source of the biofluid in fluid communication with the at least one inlet of the at least one microfluidic separation channel;
a source of an additive in fluid communication with the source of the biofluid, configured to introduce at least one additive into the biofluid, the additive capable of altering at least one of size of the target particles, size of the non-target particles, compressibility of the biofluid, compressibility of the target particles, compressibility of the non-target particles, aggregation potential of the target particles, aggregation potential of the non-target particles, density of the biofluid, density of the target particles, and density of the non-target particles;
at least one acoustic transducer coupled to a wall of the at least one microfluidic separation channel;
a recycle line extending from the second collection channel in fluid communication with the source of the biofluid, configured to recycle target particle depleted fluid from the second outlet to the source of the biofluid;
at least one sensor configured to measure at least one of density of the biofluid and concentration of target particles or non-target particles; and
a control module in electrical communication with the at least one sensor and the source of the additive, configured to introduce a predetermined volume of the additive into the biofluid in response to a measurement of at least one of the density of the biofluid and the concentration of the target particles or the non-target particles in the biofluid.