US 11,835,510 B2
Obtaining information from a biological sample in a flow cell
Tarun Khurana, Freemont, CA (US); Ali Agah, Menlo Park, CA (US); Aathavan Karunakaran, Berkeley, CA (US); and Xi-Jun Chen, Belmont, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Sep. 9, 2021, as Appl. No. 17/470,228.
Application 17/470,228 is a division of application No. 17/254,473, granted, now 11,137,385, previously published as PCT/US2020/034514, filed on May 26, 2020.
Claims priority of provisional application 62/855,622, filed on May 31, 2019.
Prior Publication US 2021/0405019 A1, Dec. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/00 (2006.01); G01N 33/483 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); A61K 39/12 (2006.01)
CPC G01N 33/4836 (2013.01) [G01N 33/5047 (2013.01); G01N 33/5308 (2013.01); A61K 39/12 (2013.01); G01N 2500/10 (2013.01)] 21 Claims
 
1. A method comprising:
contacting a biological sample with an apparatus, the apparatus comprising:
(a) a flow cell body defining one or more flow channels and a plurality of wells, each flow channel of the one or more flow channels to receive a flow of fluid, each well of the plurality of wells being fluidically coupled with the corresponding flow channel of the one or more flow channels, each well of the plurality of wells defining a corresponding depth,
(b) a plurality of electrodes, each electrode of the plurality of electrodes being positioned in a corresponding well of the plurality of wells, the plurality of electrodes to effect one or both of reading or writing of a polynucleotide in the corresponding well of the plurality of wells, and
(c) an imaging assembly to capture images indicative of the nucleotide in a polynucleotide written in at least one well of the plurality of wells;
obtaining information by one or both of reading or writing of said polynucleotide;
wherein said contacting comprises contacting said biological sample with the flow cell body of said apparatus;
wherein said information is a DNA or RNA sequence contained within said biological sample;
the method further comprising:
storing said obtained information on or in a biological cell; and
storing said biological cell with said obtained information stored on or in the biological cell.