US 11,834,714 B2
Detection and digital quantitation of multiple targets
Eleen Yee Lam Shum, San Carlos, CA (US); Hei Mun Christina Fan, Palo Alto, CA (US); Stephen P. A. Fodor, Palo Alto, CA (US); Janice Hoiyi Lai, Mountain View, CA (US); Jung Won Keum, Palo Alto, CA (US); and Haeun Grace Lee, Palo Alto, CA (US)
Assigned to ENUMERIX, INC., Palo Alto, CA (US)
Filed by Enumerix, Inc., Palo Alto, CA (US)
Filed on Dec. 20, 2022, as Appl. No. 18/085,217.
Application 18/085,217 is a continuation of application No. PCT/US2022/053413, filed on Dec. 19, 2022.
Claims priority of provisional application 63/291,813, filed on Dec. 20, 2021.
Prior Publication US 2023/0193385 A1, Jun. 22, 2023
Int. Cl. C12Q 1/6876 (2018.01); G01N 21/64 (2006.01); G01N 33/542 (2006.01)
CPC C12Q 1/6876 (2013.01) [G01N 21/6428 (2013.01); G01N 33/542 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01); G01N 2021/6439 (2013.01)] 22 Claims
 
1. A method comprising:
performing a digital multiplexed analysis of a sample distributed across a set of partitions comprising more than 500,000 partitions retained in position along three axes within a closed container, wherein upon performing a set of processes, the digital multiplexed analysis has a signal-to-noise ratio (SNR) greater than 104, and wherein the set of processes comprises:
reacting the sample with the set of processing materials within the set of partitions, and
detecting signals from the set of partitions upon performing 3D scanning of the set of partitions with a number of color channels,
wherein said signals correspond to a set of color combinatorics paired with targets of a set of targets potentially represented in the sample and contained within partitions of the set of partitions, and wherein the set of targets has a total number greater than the number of color channels used to detect colors corresponding to the set of color combinatorics, and wherein the number of color channels comprises at least 4 color channels.