US 11,834,711 B2
Sample preparation methods, systems and compositions
Timothy A Blauwkamp, Palo Alto, CA (US); Rene Sit, Sunnyvale, CA (US); and Igor D. Vilfan, Palo Alto, CA (US)
Assigned to Karius, Inc., Redwood City, CA (US)
Filed by Karius, Inc., Redwood City, CA (US)
Filed on Sep. 24, 2021, as Appl. No. 17/484,674.
Application 15/930,488 is a division of application No. 15/952,203, filed on Apr. 12, 2018, granted, now 10,697,008, issued on Jun. 30, 2020.
Application 17/484,674 is a continuation of application No. 15/930,488, filed on May 13, 2020, granted, now 11,180,800.
Claims priority of provisional application 62/484,856, filed on Apr. 12, 2017.
Prior Publication US 2022/0186304 A1, Jun. 16, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); C12N 9/00 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/6806 (2018.01); C12N 9/12 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6876 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12N 9/1247 (2013.01); C12N 9/93 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6876 (2013.01); C12Q 2521/107 (2013.01); C12Q 2533/101 (2013.01); C12Y 207/07006 (2013.01); C12Y 207/07049 (2013.01); C12Y 605/01001 (2013.01); C12Y 605/01003 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method for concurrent processing of different nucleic acid forms in a sample comprising:
(a) providing a sample comprising a first nucleic acid form and a second nucleic acid form;
(b) denaturing said first nucleic acid form and said second nucleic acid form;
(c) ligating a first adapter to one end of said first nucleic acid form using a first ligase that has a preference for said first nucleic acid form and ligating a second adapter to one end of said second nucleic acid form using a second ligase that has a preference for said second nucleic acid form, wherein said first adapter and said second adapter each comprise an identifying sequence that is different from each other; and
(d) detecting said ligated nucleic acid forms.