	US 11,834,699 B2
	Methods of nucleic acid sequencing
Niall Anthony Gormley, Cambridge (GB); Louise Fraser, Cambridge (GB); and Paula Kokko-Gonzales, Cambridge (GB)
Assigned to Illumina Cambridge Limited, Cambridge (GB)
Filed by ILLUMINA CAMBRIDGE LIMITED, Cambridge (GB)
Filed on Apr. 1, 2019, as Appl. No. 16/372,186.
Application 16/372,186 is a continuation of application No. 15/421,979, filed on Feb. 1, 2017, granted, now 10,267,804.
Application 15/421,979 is a continuation of application No. 14/378,613, granted, now 9,574,226, issued on Feb. 21, 2017, previously published as PCT/EP2013/054517, filed on Mar. 6, 2013.
Claims priority of provisional application 61/607,418, filed on Mar. 6, 2012.
Prior Publication US 2019/0324042 A1, Oct. 24, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6869 (2018.01)

CPC C12Q 1/6806 (2013.01) [C12Q 1/6834 (2013.01); C12Q 1/6869 (2013.01)]

18 Claims

1. A composition comprising

a DNA:RNA duplex immobilized on a support, wherein the duplex is immobilized on the support via an immobilized primer, and wherein at least one strand of the duplex comprises a sequence complementary to at least a portion of the immobilized primer; and

a transposome complex, wherein the transposome complex comprises a transposase and a transposon composition,

wherein the transposon composition comprises a double-stranded nucleic acid comprising a transferred strand comprising a first transposon sequence and a non-transferred strand comprising a second transposon sequence that is complementary to the first transposon sequence.