US 11,834,697 B2
Electrochemical recognition and quantification of cytochrome c oxidase expression in bacteria
Richard Guy Compton, Oxford (GB); and Sabine Kuss, Oxford (GB)
Assigned to OXFORD UNIVERSITY INNOVATION LIMITED, Oxford (GB)
Appl. No. 16/644,539
Filed by OXFORD UNIVERSITY INNOVATION LIMITED, Oxford (GB)
PCT Filed Sep. 17, 2018, PCT No. PCT/GB2018/052642
§ 371(c)(1), (2) Date Mar. 5, 2020,
PCT Pub. No. WO2019/053467, PCT Pub. Date Mar. 21, 2019.
Claims priority of application No. 1714928 (GB), filed on Sep. 15, 2017.
Prior Publication US 2020/0199644 A1, Jun. 25, 2020
Int. Cl. C12Q 1/26 (2006.01); C12Q 1/02 (2006.01); G01N 27/327 (2006.01); G01N 27/416 (2006.01); G01N 27/48 (2006.01)
CPC C12Q 1/26 (2013.01) [C12Q 1/025 (2013.01); G01N 27/3277 (2013.01); G01N 27/4161 (2013.01); G01N 27/48 (2013.01); G01N 2333/90216 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of determining the presence of bacteria expressing cytochrome c oxidase (‘the bacteria’), the method comprising:
providing a liquid sample suspected of containing the bacteria;
providing a compound that has two redox states: a reduced state and an oxidised state, wherein cytochrome c oxidase can convert the compound from its reduced state to its oxidised state;
contacting an electrode either with
(i) the compound in its oxidised state in the presence of the liquid sample, then applying a reductive potential and measuring the current at the electrode; or
(ii) the compound in its reduced state in the presence of the liquid sample, then applying an oxidative potential and measuring the current at the electrode; and
comparing the magnitude of the current produced by the reductive potential or oxidative potential in the presence of the liquid sample suspected of containing the bacteria with the magnitude of the current produced under the same conditions, but in the absence of the liquid sample suspected of containing the bacteria,
wherein a difference between the magnitude of current produced in the presence of the liquid sample suspected of containing the bacteria and the magnitude of current produced in the absence of the liquid sample suspected of containing the bacteria indicates the presence of the bacteria.