	US 11,834,671 B2
	Method of producing cancer stem cells
Shinya Tanaka, Hokkaido (JP); Kazunori Yasuda, Hokkaido (JP); Jian Ping Gong, Hokkaido (JP); Masumi Tsuda, Hokkaido (JP); and Takayuki Kurokawa, Hokkaido (JP)
Assigned to National University Corporation Hokkaido University
Appl. No. 16/487,247
Filed by National University Corporation Hokkaido University, Hokkaido (JP)
PCT Filed Feb. 20, 2018, PCT No. PCT/JP2018/005884 § 371(c)(1), (2) Date Dec. 6, 2019, PCT Pub. No. WO2018/151309, PCT Pub. Date Aug. 23, 2018.
Claims priority of application No. 2017-028833 (JP), filed on Feb. 20, 2017.
Prior Publication US 2020/0095541 A1, Mar. 26, 2020
Int. Cl. C12N 5/00 (2006.01); C12N 5/095 (2010.01); G01N 33/50 (2006.01); C12Q 1/02 (2006.01)

CPC C12N 5/0068 (2013.01) [C12N 5/0018 (2013.01); C12N 5/0695 (2013.01); C12Q 1/02 (2013.01); G01N 33/5073 (2013.01); C12N 2500/50 (2013.01); C12N 2502/1352 (2013.01); C12N 2533/30 (2013.01); C12N 2535/00 (2013.01)]

8 Claims

1. A method of producing cancer stem cells from brain, lung or ovarian cancer tissue, comprising:

culturing a living cell population from said cancer tissue in the presence of a gel substance to obtain a living cell population containing brain, lung or ovarian cancer stem cells,

wherein said gel substance is a synthetic polymer gel that induces expression of osteopontin in cells present in the living cell population obtained from cancer tissue, and converts at least a portion of said cells contained in the living cell population in to cancer stem cells; and

said synthetic polymer gel is composed of poly(sodium p-styrene sulfonate) (PNaSS) gel, poly-N-(carboxymethyl-N,N-dimethyl-2-(methacryloyloxy) ethanaminium (PCDME) gel, polyampholyte (PA) gel, poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS) gel, poly(N,N′-dimethylacrylamide) (PDMA) gel, poly(acrylic acid) (PAAc) gel, or double network gel having an interpenetrating network structure of PAMPS gel and PDMA gel.