US 11,833,526 B2
Background defocusing and clearing in ferrofluid-based capture assays
Hur Koser, Wallingford, CT (US)
Assigned to Ancera Inc., Branford, CT (US)
Filed by Ancera Inc., Branford, CT (US)
Filed on Mar. 25, 2022, as Appl. No. 17/704,820.
Application 17/704,820 is a continuation of application No. 15/739,466, granted, now 11,285,490, previously published as PCT/US2016/039394, filed on Jun. 24, 2016.
Claims priority of provisional application 62/185,534, filed on Jun. 26, 2015.
Prior Publication US 2022/0212201 A1, Jul. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B03C 1/253 (2006.01); B03C 1/023 (2006.01); B03C 1/32 (2006.01)
CPC B03C 1/253 (2013.01) [B03C 1/023 (2013.01); B03C 1/32 (2013.01); B01L 2400/043 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for extracting target particles from a ferrofluid, the method comprising:
receiving a flow within a microchannel;
generating a first magnetic field corresponding to a focusing excitation, the first magnetic field generated by a plurality of electrodes arranged proximate the microchannel,
wherein the focusing excitation is configured to focus the flow of a plurality of target particles to a surface of a capture region,
capturing the plurality of target particles on the surface of the capture region;
generating a second magnetic field corresponding to a defocusing excitation, the defocusing excitation configured to remove unbound particles from the capture region without removing target particles bound to the capture molecules;
and
detecting the bound target particles via a detector.