	US 11,833,272 B2
	Silk-fibroin hydrogels, methods of forming, and uses thereof
Fiorenzo G. Omenetto, Lexington, MA (US); Matthew B. Applegate, Somerville, MA (US); and David L. Kaplan, Concord, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Appl. No. 16/063,479
Filed by Trustees of Tufts College, Medford, MA (US)
PCT Filed Dec. 16, 2016, PCT No. PCT/US2016/067269 § 371(c)(1), (2) Date Jun. 18, 2018, PCT Pub. No. WO2017/123383, PCT Pub. Date Jul. 20, 2017.
Claims priority of provisional application 62/268,993, filed on Dec. 17, 2015.
Prior Publication US 2020/0069845 A1, Mar. 5, 2020
Int. Cl. A61L 27/52 (2006.01); A61L 27/48 (2006.01)

CPC A61L 27/52 (2013.01) [A61L 27/48 (2013.01); A61L 2400/06 (2013.01); A61L 2430/16 (2013.01)]

10 Claims

1. A method, comprising:

providing a mixture comprising silk fibroin and a flavin compound;

contacting the mixture to corneal tissue; and

exposing the mixture to visible light to induce dityrosine crosslinks in the silk fibroin to form a silk fibroin hydrogel that adheres to the corneal tissue.