US 11,833,192 B2
Method for improving intestinal flora
Manabu Kuroda, Kakamigahara (JP); Shotaro Yamaguchi, Kakamigahara (JP); and Norihisa Kato, Higashihiroshima (JP)
Assigned to AMANO ENZYME INC., Nagoya (JP); and HIROSHIMA UNIVERSITY, Higashihiroshima (JP)
Filed by AMANO ENZYME INC., Nagoya (JP); and HIROSHIMA UNIVERSITY, Higashihiroshima (JP)
Filed on Oct. 5, 2021, as Appl. No. 17/494,733.
Application 17/494,733 is a division of application No. 15/999,357, granted, now 11,167,016, previously published as PCT/JP2017/005986, filed on Feb. 17, 2017.
Claims priority of application No. 2016-028942 (JP), filed on Feb. 18, 2016.
Prior Publication US 2022/0023396 A1, Jan. 27, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/62 (2006.01); A61K 38/48 (2006.01); A61P 1/14 (2006.01); A23L 33/17 (2016.01); A61P 1/10 (2006.01); A61K 9/00 (2006.01); A61K 38/46 (2006.01); A23L 2/52 (2006.01); A61K 36/062 (2006.01); C12N 9/50 (2006.01); A23L 33/10 (2016.01); A23L 29/00 (2016.01); A23K 50/30 (2016.01); C12N 9/26 (2006.01); C12N 9/24 (2006.01)
CPC A61K 38/46 (2013.01) [A23K 50/30 (2016.05); A23L 2/52 (2013.01); A23L 29/06 (2016.08); A23L 33/10 (2016.08); A23L 33/17 (2016.08); A61K 9/0053 (2013.01); A61K 36/062 (2013.01); A61K 38/48 (2013.01); A61K 38/4806 (2013.01); A61P 1/10 (2018.01); A61P 1/14 (2018.01); C12N 9/248 (2013.01); C12N 9/2411 (2013.01); C12N 9/50 (2013.01); C12N 9/62 (2013.01); A01K 2227/108 (2013.01); A23V 2002/00 (2013.01)] 2 Claims
 
1. A method for improving intestinal flora, comprising orally applying a protease comprising at least one of the following polypeptides (1) to (3) to a person who requires improvement of intestinal flora:
(1) a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 1;
(2) a polypeptide comprising an amino acid sequence in which one or a few amino acids are substituted, added, inserted, or deleted in the amino acid sequence shown in SEQ ID NO: 1, and having a protease activity equivalent to that of a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 1; and
(3) a polypeptide comprising an amino acid sequence having 80% or more sequence identity to an amino acid sequence shown in SEQ ID NO: 1, and having a protease activity equivalent to that of a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 1,
wherein the protease increase the number of Bifidobacteria and Lactic acid bacteria in intestines of said subject, thereby improves the intestinal flora.